Catechin이 Porphyromonas Gingivalis세균외막의 물리적 특성에 미치는 영향
The Effect of Catechin on Physical Properties of Outer Membranes Cultured from Porphyromonas Gingivalis
성기수, 김태영, 주지민, 김다솔, 장혜옥,
소속 상세정보
성기수 ( Seong Ki-Soo ) -
김태영 ( Kim Tae-Young ) -
주지민 ( Ju Ji-Min ) -
김다솔 ( Kim Da-Sol ) -
장혜옥 ( Jang Hye-Ock ) -
Abstract
Fluorescent probe were used to evaluate the effects of catechin on the structural parameters (annular lipid fluidity, transbilayer lateral and rotational mobility and protein clustering) of the Porphyromonas gingivalis outer membrane (OPGs). An experimental procedure was used on the basis of selective quenching of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups and radiationless energy transfer from the tryptophans of membrane proteins to Py-3-Py and DPH. Catechin increased the bulk lateral and rotational mobility, annular lipid fluidity of OPGs lipid bilayers, and had greater fluidizing efficacy on the outer monolayer than the inner monolayer. It also caused membrane proteins to cluster. Based on these effects of catechin on OPGs, the antibacterial and antiviral actions of catechin can be partially explained.
키워드
Bacterial outer membranes; Catechin; Fluorescence probe technique; Lateral mobility; Rotational mobility
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